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HB Electrophoresis (HPLC) Test in Hyderabad

Also Known as Abnormal Hb Studies (Hb Electrophoresis), Hb Electrophoresis, HPLC, Abnormal Hb Studies

No preparation required

MRP 3500 60% off
1400

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RECOMMENDED BY:Dr. Bhattacharya

Specialises in Blood Studies

testsTest(s) Included (19)

  • HB Electrophoresis (HPLC) Test

    19 tests included

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*Optional Tests: Testing of these is conditional depending on results of other tests

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BLOOD

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AGE GROUP

7 years & above

HB Electrophoresis (HPLC) Test in Hyderabad

Overview of the HB Electrophoresis (HPLC) Test

Test Overview

Sample TypeBlood
Reports DeliveryReports available within 5 days
Price/Cost1400
Number of Tests Included19

Haemoglobin, a vital protein found in red blood cells, is responsible for carrying oxygen throughout the body. An abnormal Hb study analyses the different types of haemoglobin within the bloodstream. Haemoglobin variants can be inherited, and detecting them early can aid in managing and treating related health issues. The test separates different types of haemoglobin based on their electrical charge, allowing for a detailed analysis of an individual's haemoglobin composition.

Doctors often recommend the hemoglobin electrophoresis test for detecting abnormal variants of haemoglobin, which can indicate conditions such as anaemia, thalassaemia, or sickle cell anaemia. For individuals planning to start a family, this test is crucial in assessing whether they are carriers for such conditions.

Early detection through hemoglobin electrophoresis test allows for better management of potential health risks, ensuring appropriate medical care and genetic counselling. Additionally, this test is valuable in monitoring patients with known haemoglobin disorders, guiding treatment decisions, and assessing disease progression.

Tests Included in HB Electrophoresis (HPLC) Test in Hyderabad

Knowing the components of an HPLC blood test is crucial for accurately assessing and managing haemoglobin-related conditions. Below are the key components included in the Hb electrophoresis test list:

  • Foetal Haemoglobin, Quantitative: Measures the amount of foetal haemoglobin (HbF) in the blood, which can indicate certain blood disorders like thalassaemia or hereditary persistence of foetal haemoglobin.
  • Haemoglobin A (HbA): The most common type of haemoglobin in adults, accounting for about 95-98% of the haemoglobin. It consists of two alpha and two beta chains.
  • Haemoglobin A2 (HbA2): A minor component of adult haemoglobin, normally making up about 2-3% of total haemoglobin. Elevated levels can indicate beta-thalassaemia.
  • Haemoglobin C (HbC): A variant of normal haemoglobin A. Individuals with haemoglobin C trait have both normal and abnormal haemoglobin. Haemoglobin C disease can cause mild haemolytic anaemia.
  • Haemoglobin D (HbD): A rare haemoglobin variant. Individuals with haemoglobin D trait usually have no symptoms, but haemoglobin D disease can cause mild anaemia and splenomegaly i.e. enlargement of the spleen.
  • Haemoglobin S (HbS): The abnormal haemoglobin in sickle cell disease. It causes red blood cells to become rigid, sticky, and shaped like sickles, leading to various complications.
  • Haemoglobin E (HbE): A common haemoglobin variant in Southeast Asia. Haemoglobin E trait is usually benign, but haemoglobin E disease can cause mild anaemia and microcytosis.
  • P2 Window: A specific part of the hemoglobin electrophoresis test that helps in identifying and quantifying different types of haemoglobin present in a sample.
  • P3 Window: Another part of the hemoglobin electrophoresis test. It aids in the detailed analysis and detection of minor haemoglobin variants.
  • Haemoglobin: The protein in red blood cells that carries oxygen from the lungs to the rest of the body. Its levels are measured to diagnose anaemia and other blood disorders.
  • RBC Count: Measures the number of red blood cells in a volume of blood. It helps diagnose conditions like anaemia, dehydration, and polycythaemia.
  • PCV (Packed Cell Volume): Also known as haematocrit, it measures the proportion of blood volume occupied by red blood cells. It helps diagnose anaemia and other hematologic conditions.
  • MCH (Mean Corpuscular Haemoglobin): The average amount of haemoglobin in a single red blood cell. It helps diagnose the cause of anaemia, distinguishing between microcytic and macrocytic anaemia.
  • MCHC (Mean Corpuscular Haemoglobin Concentration): The average concentration of haemoglobin in a given volume of red blood cells. It is used to diagnose and classify different types of anaemia.
  • MCV (Mean Corpuscular Volume): The average volume of a red blood cell. It helps determine the type of anaemia (microcytic, normocytic, or macrocytic).
  • RDW (Red Cell Distribution Width): Measures the variation in red blood cell size and volume. Elevated RDW can indicate mixed anaemia or anisocytosis i.e. variation in red blood cell size.
  • Peripheral Smear Finding: A microscopic examination of blood smear. It helps identify abnormalities in the size, shape, and number of blood cells, aiding in the diagnosis of various hematologic conditions.

What Conditions Can Be Diagnosed With the HB Electrophoresis (HPLC) Test?

The HB Electrophoresis (HPLC) Test is primarily used to diagnose haemoglobin disorders by detecting abnormal haemoglobin variants. It helps identify conditions such as:

  • Sickle Cell Disease and Trait (HbS): A genetic disorder causing abnormal, sickle-shaped red blood cells.
  • Thalassaemia (Alpha and Beta): A group of inherited blood disorders leading to reduced or absent haemoglobin production.
  • Haemoglobin C, D, and E Disorders: Genetic conditions that can cause mild to moderate anaemia.
  • Hereditary Persistence of Foetal Haemoglobin (HPFH): A condition where foetal haemoglobin (HbF) remains in significant amounts into adulthood.
  • Anaemia Diagnosis and Classification: Helps differentiate between iron-deficiency anaemia, haemolytic anaemia, and inherited anaemias.

Test Requirements

Proper Hb electrophoresis test preparation is crucial to ensure precise and accurate test outcomes essential for subsequent diagnosis.

A. How is the HB Electrophoresis (HPLC) Test Done?

The hemoglobin electrophoresis test is a straightforward and fast procedure where a blood sample is taken from a vein in your arm.

  • A doctor or trained healthcare professional will draw a small amount of blood from a vein in your arm using a fine needle. This blood sample is then collected in a vial or test tube for analysis. The collected blood sample will be sent to a laboratory where the different types of haemoglobin in the serum will be evaluated.
  • While you might feel a slight sting as the needle pierces your skin for the blood sample, the process is usually quick, typically lasting less than five minutes.
  • Occasionally, you may experience some pain or bruising at the site where the needle was inserted, but these symptoms resolve quickly.

B. Is Fasting Required for the HB Electrophoresis (HPLC) Test?


Fasting is typically not required for an HPLC blood test.

  • Your healthcare provider may recommend temporary cessation or adjustment of certain medications to avoid interference with the test.

It's always best to follow the advice of your family physician for the Hb electrophoresis test preparation to ensure accurate results.

C. What Time of the Day Should I Undergo an HB Electrophoresis (HPLC) Test?

One crucial aspect to consider when undergoing the Hb electrophoresis test is timing. Individuals can undergo the test at any time of the day.

  • Most doctors recommend taking this test early in the morning at an ESR lab. Early appointments help ensure accurate results and timely diagnosis.

However, always consult your doctor about timings as they may vary based on personal health conditions and other factors.

Price of the HB Electrophoresis (HPLC) Test in Hyderabad

Abnormal Hb Studies Test Price in Different Cities

CityAbnormal Hb Studies Test Price
Bangalore₹1811
Hyderabad₹1811
Chennai₹1487
Gurgaon₹1487
Delhi₹1487

The hemoglobin electrophoresis test is generally affordable, but understanding the key factors that influence the Hb electrophoresis test price can assist in managing healthcare expenses effectively. Below are the primary factors affecting the Hb electrophoresis test cost:

  • Laboratory Standards: The quality of the laboratory conducting the Hb electrophoresis test procedure influences the overall cost. Laboratories adhering to international standards and employing advanced technology with strict quality controls typically charge higher prices. Choosing accredited labs known for their reliability is crucial.
  • Comprehensive Inclusions: The HPLC blood test price generally includes several components, such as laboratory fees, sample collection charges, and any other related professional fees. Additionally, information about the Hb electrophoresis test normal range may also be provided, giving you a comprehensive understanding of your test results. Knowing what these charges include ensures transparency and helps avoid unexpected expenditures.
  • Discounts and Offers: Healthcare facilities frequently offer discounted rates or package deals for diagnostic tests such as the HPLC blood test, which substantially lowers the overall test prices.

Apollo 24|7 maintains competitive Hb electrophoresis test prices while ensuring high service standards. Before scheduling an HPLC blood test, it's advisable to compare prices across different locations.

Reports of the HB Electrophoresis (HPLC) Test

Information about the Hb electrophoresis test normal range is crucial for preventive healthcare, aiding in the early identification of potential health risks. Below are the test ranges:

TestNormal RangeHigh RangeLow Range
Foetal Haemoglobin (HbF)<2% in adults>2%Not typically a concern
Haemoglobin A (HbA)95-98% of total haemoglobinNot typically a concern<95%
Haemoglobin A2 (HbA2)2-3.5% of total haemoglobin>3.5%<2%
HaemoglobinMen: 13.8-17.2 g/dL
Women: 12.1-15.1 g/dL
Men: >17.2 g/dL
Women: >15.1 g/dL
Men: <13.8 g/dL
Women: <12.1 g/dL
RBC CountMen: 4.7-6.1 million cells/µL
Women: 4.2-5.4 million cells/µL
Men: >6.1 million cells/µL
Women: >15.1 g/dL
Men: <4.7 million cells/µL
Women: <4.2 million cells/µL
PCV (Packed Cell Volume)Men: 40-54%
Women: 36-48%
Men: >54%
Women: >48%
Men: <40%
Women: <36%
MCH (Mean Corpuscular Haemoglobin)27-33 picograms/cell>33 pg/cell<27 pg/cell
MCHC (Mean Corpuscular Haemoglobin Concentration)32-36 g/dL>36 g/dL<32 g/dL
MCV (Mean Corpuscular Volume)80-100 femtolitres>100 fL<80 fL
RDW (Red Cell Distribution Width)11.5-14.5%>14.5%Not typically a concern
TestNormal FindingsAbnormal Findings
Haemoglobin C (HbC)Not presentPresent
Haemoglobin D (HbD)Not presentPresent
Haemoglobin S (HbS)Not presentPresent
Haemoglobin E (HbE)Not presentPresent
P2 WindowNot applicableDeviations indicate the presence of abnormal haemoglobins
P3 WindowNot applicableDeviations indicate the presence of abnormal haemoglobins
Peripheral Smear FindingNormal size, shape, and appearance of red blood cells (RBCs), white blood cells (WBCs), and platelets.Changes in RBC size, shape, or colour.

Abnormal WBC types or numbers.

Abnormal platelet numbers.

Presence of immature or abnormal cell types

The condition or disease indicated by abnormal levels are given below:

TestHigh Value IndicatesLow Value Indicates
Foetal Haemoglobin (HbF)Thalassaemia which is an inherited blood disorder affecting haemoglobin production.

Sickle Cell Disease, a genetic condition causing abnormal haemoglobin, leading to sickle-shaped red blood cells. Hereditary Persistence of HbF: Genetic condition where foetal haemoglobin persists into adulthood.
Not typically a concern
Haemoglobin A (HbA)Not typically a concernHaemoglobinopathies, i.e. genetic disorders affecting haemoglobin synthesis.

Beta-Thalassaemia: Inherited blood disorder resulting in reduced production of beta-globin chains.
Haemoglobin A2 (HbA2)Beta-Thalassaemia TraitIron Deficiency Anaemia: Lack of iron leading to reduced haemoglobin production.

Alpha-Thalassaemia which is a deficiency in alpha globin chains of haemoglobin.
HaemoglobinPolycythaemia i.e. increased red blood cell countAnaemia i.e. decreased red blood cell count or haemoglobin levels.
RBC CountPolycythaemiaAnaemia
PCV (Packed Cell Volume)PolycythaemiaAnaemia
MCH (Mean Corpuscular Haemoglobin)Macrocytic anaemia wherein red blood cells are larger than normal.Microcytic anaemia wherein red blood cells are smaller than normal.
MCHC (Mean Corpuscular Haemoglobin Concentration)Spherocytosis: Genetic disorder causing spherical-shaped red blood cells.

Hypochromic Anaemia i.e. red blood cells with low haemoglobin content.

Iron Deficiency Anaemia

RDW (Red Cell Distribution Width)

Mixed anaemia

Anisocytosis i.e. variation in red blood cell size.

Not typically a concern
TestPresence Indicates
Haemoglobin C (HbC)Sickle Cell Disease with HbC, the individuals have both sickle cell trait (HbS) and haemoglobin C trait (HbC), resulting in varying symptoms of anaemia and potential complications.

Haemoglobin C Disease, an inherited blood disorder where the beta-globin protein is abnormal due to a mutation leading to mild chronic haemolytic anaemia.
Haemoglobin D (HbD)Haemoglobin D Disorders: It is a genetic condition where haemoglobin D variants (HbD) are present, causing varying degrees of haemolytic anaemia depending on the specific mutation.

Sickle Cell Disease with HbD: Coexistence of sickle cell trait (HbS) and haemoglobin D trait (HbD), leading to symptoms of sickle cell disease with additional characteristics of haemoglobin D disorders.
Haemoglobin S (HbS)Sickle Cell Disease, Sickle Cell Trait
Haemoglobin E (HbE)Haemoglobin E disorders, a condition involving haemoglobin E variant (HbE), which can lead to mild to moderate anaemia and may combine with beta-thalassaemia to cause more severe symptoms.

Beta-Thalassaemia with HbE: Combination of beta-thalassaemia (reduced or absent beta-globin production) with haemoglobin E variant, causing anaemia and other related symptoms.
P2 WindowVarious abnormal haemoglobinopathies which is a broad category encompassing genetic disorders affecting haemoglobin structure or production, leading to abnormal haemoglobin patterns on electrophoresis
P3 WindowVarious abnormal haemoglobinopathies
Peripheral Smear FindingAbnormal peripheral smear findings, such as microcytic and hypochromic red blood cells, suggest conditions like iron deficiency anaemia and thalassemia.

The Presence of sickle-shaped RBCs indicates sickle cell disease, while immature RBCs (reticulocytes) and abnormal white blood cells (blasts) point to haemolytic processes, leukaemia, or infections like malaria.

Thrombocytopenia denotes decreased platelet counts.

How Often Should You Be Recommended HB Electrophoresis (HPLC) Test?

The frequency of the HB Electrophoresis (HPLC) Test depends on individual health factors, medical history, and genetic risks. It is primarily used for diagnosing and monitoring haemoglobin disorders, making it essential for certain groups of people. Below are the key recommendations for when you should get tested:

  • For couples planning a family: Before pregnancy to detect potential haemoglobin disorders.
  • For individuals with a family history of blood disorders: Once in a lifetime to assess genetic risk.
  • For those experiencing persistent anaemia: As advised by a doctor based on symptoms and prior test results.
  • For patients diagnosed with haemoglobin disorders: Annually or more frequently to monitor disease progression.
  • For individuals undergoing regular blood transfusions: Before each transfusion to ensure compatibility and effective management.

Book the HB Electrophoresis (HPLC) Test Online

Online healthcare services offer significant convenience, particularly beneficial for elderly individuals, pregnant women, and those with restricted mobility. This section provides a step-by-step outline for booking the Hb electrophoresis test online through Apollo 24|7 and accessing the test report once it becomes available.

A. What are the Steps to Schedule the HB Electrophoresis (HPLC) Test on Apollo 24|7?

Booking a hemoglobin electrophoresis test via Apollo 24|7 is simple and convenient. The process is user-friendly and can be completed swiftly from the comfort of your own home. Below are the steps involved:

  1. Visit Apollo 24|7 Website or App: To initiate the process, access the official Apollo 24|7 website or install their app on your mobile device. The design is intuitive and optimised for easy navigation.
  2. Search for the Test: On reaching the homepage, locate the search box, typically at the top of the page. Type in "hemoglobin electrophoresis test". This will take you directly to the page dedicated to this crucial blood test.
  3. Choose a Convenient Time and Location: Now that you're on the hemoglobin electrophoresis test page, you can select a time slot and location that fits your schedule for your blood sample collection. You can also look up Hb electrophoresis test procedure near your location to find the relevant details.
  4. Complete Your Booking: Once you've chosen your preferred time and location, proceed to complete your booking by entering all required details.
  5. Wait for Confirmation: After you've placed an order for your Hb electrophoresis test procedure, simply wait for a confirmation message from Apollo 24|7. This last step ensures that your request has been accurately processed.

B. How do I Access my HB Electrophoresis (HPLC) Test Results Online on Apollo 24|7?

Once your blood sample has been collected, follow these simple steps to access, review, and download your Hb electrophoresis test report through the Apollo 24|7 platform:

  1. Await Notification: Apollo 24|7 will send you a notification when your test results are ready for viewing.
  2. Log into Your Account: Access your account on the Apollo 24|7 website or mobile app using your registered login credentials.
  3. Go to the Reports Section: Upon successful login, navigate to the designated 'Reports' section where all your medical test results are stored.
  4. Find 'Erythrocyte Sedimentation Rate Test': Locate the specific entry for your hemoglobin electrophoresis test within the available reports.
  5. Download Your Report: Look for the option to download and save your test report. This feature enables you to keep a copy of your Hb electrophoresis test report for future reference or to share with your doctor.
     

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faqFrequently Asked Questions (FAQs)

What is the cost of an Abnormal Hb Studies (Hb Electrophoresis) test price in Hyderabad

The Abnormal Hb Studies (Hb Electrophoresis) test price in Hyderabad cost 1400

Are there any alternative or complementary tests to consider alongside an HPLC blood test for a more comprehensive assessment?

Yes, other tests like complete blood count (CBC), iron studies, and genetic testing can provide additional information about your blood health. Your doctor may recommend these if they suspect a specific condition or want a more inclusive overview of your blood health.

Are there any emerging technologies or advancements in hemoglobin electrophoresis test testing that I should be aware of?

Molecular techniques such as DNA sequencing and next-generation sequencing are becoming more prevalent in diagnosing haemoglobin disorders. However, the HPLC blood test remains one of the most reliable methods for detecting abnormal forms of haemoglobin.

Can I drink water before an HPLC blood test?

Yes, you can drink water before undergoing an HPLC hemoglobin electrophoresis test, but you should refrain from consuming any other food or beverages.

How accurate is the hemoglobin electrophoresis test for the diagnosis of blood-related disorders?

 Hemoglobin electrophoresis tests are highly reliable at identifying abnormal types of haemoglobin which could point towards various conditions such as Thalassemia or Sickle Cell Anaemia. However, it may require additional testing for a definitive diagnosis.

How long does it take to get the results of an HPLC blood test?

Typically, laboratory results for HPLC blood tests are available within two to three days. However, this can vary depending on the laboratory performing the test and the complexity of the analysis.

What is the price of a HB Electrophoresis (HPLC) Test in Hyderabad?

The price of HB Electrophoresis (HPLC) Test at Apollo 24|7 is Rs. 1400.

How long does a HB Electrophoresis (HPLC) Test take in Hyderabad?

It takes less than 5 minutes to take HB Electrophoresis (HPLC) Test in Hyderabad. The area is cleaned using an antiseptic wipe and a tourniquet is applied to enhance blood flow before inserting a needle to collect the sample. The sample is then sent to a lab for analysis.

When will I receive my reports in Hyderabad?

The report for HB Electrophoresis (HPLC) Test is usually available within Reports available within 5 days in Hyderabad, however, the time may vary depending on the type of test. After your HB Electrophoresis (HPLC) Test is complete, you will receive a notification from Apollo 24|7 letting you know that your results are ready for viewing.

Is home sample collection available for a HB Electrophoresis (HPLC) Test in Hyderabad?

Yes, home sample collection is available for a HB Electrophoresis (HPLC) Test in Hyderabad. Moreover, you can select a suitable time slot and location for your blood sample collection.

Can I book a HB Electrophoresis (HPLC) Test near me in Hyderabad?

Yes. All you need to do is visit the Apollo 24|7 website or app and search for HB Electrophoresis (HPLC) Test. Select a convenient time and location, then finalize your booking by providing the necessary details. Wait for a confirmation message to complete the process.

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