Vitamin D (1,25 Dihydroxy Cholecalciferol) in Pragathi-enclave, Hyderabad

Vitamin D (1,25 Dihydroxy Cholecalciferol) Test: Price, Purpose, Range & Reports

Test Overview

The Vitamin D (1,25 Dihydroxy Cholecalciferol) test measures the level of 1,25-dihydroxyvitamin D (1,25(OH)2D), also known as Calcitriol. This is the biologically active hormonal form of Vitamin D, which is primarily responsible for regulating calcium, phosphate, and bone health.

Important Note: This test is not the standard screening test for general Vitamin D deficiency. The routine test is 25-Hydroxy Vitamin D (25(OH)D), which reflects the body’s total storage levels. The 1,25(OH)2D test is specifically used to investigate complex disorders related to kidney function and parathyroid hormone (PTH) issues.

What is the Vitamin D (1,25 Dihydroxy Cholecalciferol) Test?

The 1,25(OH)2D (Calcitriol) test is a blood assay that measures the final, active product of Vitamin D metabolism. Vitamin D is converted into its active form primarily in the kidneys, a step that is tightly controlled by Parathyroid Hormone (PTH) and phosphate levels.

Because its synthesis is regulated by PTH and its actions are centred on calcium homeostasis, measuring 1,25(OH)2D is critical for diagnosing and managing metabolic bone diseases, certain kidney conditions, and disorders of the parathyroid gland. When kidney function is compromised, the production of this active hormone drops significantly, even if storage levels (25(OH)D) are adequate.

What is the Vitamin D (1,25 Dihydroxy Cholecalciferol) Test Price?

The price of the Vitamin D (1,25(OH)2D) test is typically higher than the standard 25(OH)D test. This difference is due to the more complex nature of the assay required to accurately measure the low concentrations of the active hormone in the blood.

Factors Affecting the Test Cost

Complexity of Assay: The test requires high-sensitivity methods, often involving techniques like High-Performance Liquid Chromatography (HPLC) or mass spectrometry, to precisely measure the active metabolite.

• Specialised Reagents: The reagents used to specifically isolate and quantify 1,25(OH)2D are more specialised and costly.
  Regional Variation: Prices may vary slightly based on regional operating costs, laboratory accreditation (like NABL), and logistics.
  To determine the accurate and current Vitamin D (1,25(OH)2D) test price near me, please check the Apollo 24|7 app or website for real-time pricing specific to your location.

What is the Purpose of the Vitamin D (1,25(OH)2D) Test?

The primary purpose of measuring 1,25(OH)2D is to investigate disturbances in calcium and phosphate metabolism that are often linked to abnormal kidney or parathyroid function.

• Diagnosing Hypercalcemia: To investigate the cause of high calcium levels (Hypercalcemia), particularly when a condition like sarcoidosis or certain lymphomas (where 1,25(OH)2D can be produced outside the kidney) is suspected.
• Assessing Kidney Disease: To monitor patients with chronic kidney disease (CKD), as the kidney is responsible for the final activation step of Vitamin D. Low 1,25(OH)2D is expected in advanced CKD.
• Evaluating Parathyroid Disorders: To help diagnose and monitor Hyperparathyroidism (high PTH) or Hypoparathyroidism (low PTH), as PTH directly stimulates 1,25(OH)2D production.
• Diagnosing Rare Conditions: To confirm the diagnosis of certain forms of Rickets or Osteomalacia that are resistant to standard Vitamin D treatment (e.g., hereditary 1-alpha-hydroxylase deficiency).

Who Should Get the Vitamin D (1,25(OH)2D) Test Done?

This test is typically ordered by an Endocrinologist, Nephrologist, or Rheumatologist. It is generally not for the general population.
You may be advised to take this test if:

• You have high blood calcium levels (Hypercalcemia) that are not explained by primary hyperparathyroidism.
• You have a confirmed diagnosis of Chronic Kidney Disease (CKD) stage 3 or higher.
• You are being investigated for Parathyroid gland dysfunction (high or low PTH).
• You are being monitored for conditions known to cause high 1,25(OH)2D levels, such as Sarcoidosis.
• You have Rickets or Osteomalacia that is proving unresponsive to conventional 25(OH)D treatment.

Components of Vitamin D (1,25 Dihydroxy Cholecalciferol) Test

This diagnostic test is focused on quantifying the single, final hormonal metabolite of the Vitamin D pathway, which is essential for calcium and bone homeostasis.

The key analyte measured is:

• 1,25 Dihydroxy Cholecalciferol (Calcitriol): This parameter measures the concentration of the biologically active Vitamin D hormone. Its levels are critical for assessing the kidney’s ability to activate Vitamin D and for evaluating parathyroid gland function.

Understanding the Vitamin D (1,25(OH)2D) Report and Range

The report for the 1,25-Dihydroxyvitamin D test provides a quantitative measurement of the active hormone, Calcitriol. This result must be interpreted accurately and relevantly alongside other clinical and laboratory findings. The reference range for 1,25(OH)2D may vary slightly between laboratories, depending on their specific protocols, equipment, and regional guidelines. Always consult your healthcare provider for interpretation in the context of your overall health status and concurrent test results. The units are typically expressed in picograms per millilitre (pg/mL) or picomoles per litre (pmol/L).
Typical Reference Range: 18 - 72 pg/mL