Vitamin D - 25 Hydroxy (D2+D3)

Also Known as 25-Hydroxy Cholecalciferol, VITAMIN D (25-OH), VIT D TOTAL
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    blood sample




    Age group

    Above 10 years

    Vitamin D 25-hydroxy test is conducted to detect the  level of vitamin D present in your body. Vitamin D 25-hydroxy is a fat soluble vitamin, also known as calciferol.  The reason why vitamin D 25-hydroxy test is taken is to know the exact  levels of vitamin D so that the doctor can prescribe supplements according to the deficiency in the body. 

    People with a vitamin D deficiency become prone to heart diseases, high blood pressure, immune system disorders, different types of cancers (prostrate, breast and colon) and various infectious diseases. All these make it more important for any one to keep a check on their vitamin D levels. 

    Besides taking the test and consulting the doctor, one can easily keep their vitamin D level within the normal range by exposing oneself to 30 minutes of sunlight or by adding vitamin D-rich food items to their diet. Some vitamin D-rich food items are cod liver oil, tuna fish, yogurt, salmon, egg yolk, cereals and cheese. Keeping the vitamin D levels normal should be the prime aim to help the body function properly.

    Who should take a vitamin D 25-hydroxy test?  

    • People who strictly adhere to vegan diet
    • People suffering from bone pain or osteoporosis 
    • People experiencing constant backache or joint pains, especially heel pain
    • People diagnosed with depression


    Medically reviewed by Dr Shakti, General Physician/Internal Medicine at Indraprastha Apollo Hospitals, New Delhi

    faqFrequently Asked Questions (FAQs)

    Frequently asked questions

    To take the blood sample, a tourniquet (elastic) band is placed tightly on the upper arm. The patient is asked to make a fist. This helps in the buildup of blood filling the veins and it becomes easy to collect the blood. The skin is cleaned before inserting the needle in the vein in order to prevent bacteria from entering. The needle is then inserted into the vein in the arm and the blood sample is collected in the vacutainer.
    The functions of Vitamin D include regulation of blood levels of calcium, phosphorus, and magnesium. It is important for the growth and health of bone. It helps in the regulation of the immune system.
    The signs and symptoms of Vitamin D deficiency include fatigue, muscle weakness, muscle aches, muscle cramps, mood swings, bone pain, and depression.
    The effects of Vitamin D deficiency on health include heart disease, high blood pressure, diabetes, infections, immune system disorders, multiple sclerosis, cancer of colon, prostate, and breast cancers.
    Several conditions that can cause Vitamin D deficiency include cystic fibrosis, crohn’s disease, celiac disease, weight loss surgeries, obesity, kidney and liver diseases.
    Yes, please inform your doctor if you are taking any medicines or supplements as it might interfere with the test results.
    The vitamin D 25-hydroxy normal range is usually measured in nanograms per milliliter (ng/ml). It should be between 25-40 ng/ml.

    Why should Apollo be your preferred healthcare partner?

    • 40 Years of legacy and credibility in the healthcare industry.
    • NABL certified multi-channel digital healthcare platform.
    • Affordable diagnostic solutions with timely and accurate test results.
    • Up to 60% discount on Doorstep Diagnostic Tests, Home Sample Collection.
    • An inventory of over 100+ laboratories, spread across the country, operating out of 120+ cities with 1200+ collection centers, serving over 1800+ pin codes.

    The information mentioned above is meant for educational purposes only and should not be taken as a substitute to your Physician’s advice. It is highly recommended that the customer consults with a qualified healthcare professional to interpret test results