Effective price: ₹1935
Above 10 years
Anti-Saccharomyces cerevisiae antibodies (ASCA) are protein molecules present in people with inflammatory bowel disease (IBD). This disease involves several problems like significant inflammatory reactions, chronic symptoms, autoimmunity, and tissue damage.
ASCA test detects the presence of antibodies in blood because S. cerevisiae is a common yeast present in almost every food item. The ASCA blood test differentiates between Crohn’s disease and ulcerative colitis because it is difficult to assess the condition using biopsies.
Nearly 75% of people who have Crohn’s disease have ASCA in their blood and it could be either IgG or IgA. Since both Crohn’s disease and ulcerative colitis are intestinal disorders, they have similar symptoms and it gets confusing to start the treatment without proper testing.
While IgA is the first line of defence and indicates the presence of the condition in the body, IgG indicates the severity of the infection. The test also helps in finding out the type of IBD one has.
Furthermore, image diagnosis, endoscopic examination, and pathological tests fail to give a clear diagnosis of the condition. The ASCA test does not confirm the presence or absence of IBD, instead helps in differentiating two different conditions associated with bowel inflammation.
The symptoms of the condition may vary from one person to the other. Below are some common symptoms of IBD:
At Apollo 24|7, the ASCA test involves the ELISA technique which is an accurate analysis of antibodies in the body.
It takes place on a 96-well plate to avoid separate replication of the experiment. The immunoassay also observes the interaction between antigen and antibody and looks for any specific reaction.
It uses many enzymes like horseradish peroxidase, alkaline phosphatase, and beta-galactosidase. However, the key players in the reaction are antigen and antibody only. The immobilized antigen on the 96-well plate captures the primary antibody.
The next step is the identification of the captured antibody using a secondary antibody. A secondary antibody is usually a colour-producing substance that changes the colour of the mixture.
Therefore, the ELISA plate is exposed to ultraviolet rays of a specific wavelength and the intensity is read using the spectrophotometric technique. The higher the intensity of colour, the more the concentration of antibodies. If there are more antibodies present in the blood, it indicates the severity of the reaction.
Some of the advantages of going for an ELISA test are:
Why should Apollo be your preferred healthcare partner?
The information mentioned above is meant for educational purposes only and should not be taken as a substitute to your Physician’s advice. It is highly recommended that the customer consults with a qualified healthcare professional to interpret test results