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Triple Marker Test During Pregnancy

Learn about the Triple Marker Test during pregnancy, why it’s done, when to take it, what the results mean, and how it helps assess the baby’s risk for genetic conditions and birth defects.

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Written by Dr. Shaik Abdul Kalam

Reviewed by Dr. Siri Nallapu MBBS

Last updated on 2nd Sep, 2025

Triple Marker Test During Pregnancy

Pregnancy is a beautiful journey filled with excitement and anticipation. However, it also comes with the responsibility of ensuring both the mother and baby are healthy. One important test that helps monitor the baby’s well-being is the Triple Marker Test. If you’re expecting, you might have heard about this test from your doctor. But what exactly is it, and why is it done?
Let’s break it down in simple terms so you can understand its importance and what to expect.

What is the Triple Marker Test?

The Triple Marker Test, also known as the Triple Screen Test, is a blood test performed between 15 to 20 weeks of pregnancy (ideally around 16 to 18 weeks). It measures the levels of three important substances in the mother’s blood:

1. Alpha-fetoprotein (AFP) – A protein produced by the baby.
2. Human Chorionic Gonadotropin (hCG) – A hormone made by the placenta.
3. Estriol (uE3) – A form of estrogen produced by both the baby and the placenta.

These levels help doctors assess the risk of certain chromosomal abnormalities and birth defects in the baby.

Why is the Triple Marker Test Done?

The test is not diagnostic, meaning it doesn’t confirm a condition but helps estimate the risk of certain problems. It screens for:

  • Down Syndrome (Trisomy 21) – A genetic disorder causing developmental delays.
  • Edwards Syndrome (Trisomy 18) – A severe chromosomal disorder affecting growth.
  • Neural Tube Defects (NTDs) – Problems in the baby’s brain or spine (like spina bifida).

Who Should Get This Test?

While the test is optional, doctors may recommend it if:

  • You are 35 years or older (higher risk of chromosomal issues).
  • You have a family history of genetic disorders.
  • Previous pregnancies had birth defects.
  • You had abnormal ultrasound findings.

However, even if you don’t fall into these categories, you can still opt for the test for reassurance.

How is the Test Done?

The test is simple and safe:

1. A small blood sample is taken from your arm.
2. The sample is sent to a lab for analysis.
3. Results are usually available in a few days.
No fasting or special preparation is needed!

Consult a Gynaecologist for Personalised Advice

Dr. Priyanka Surisetty, Obstetrician and Gynaecologist

Dr. Priyanka Surisetty

Obstetrician and Gynaecologist

8 Years • MBBS, DGO

Visakhapatnam

Apollo 24|7 Clinic - Andhra Pradesh, Visakhapatnam

660

Dr Swatika Kumari, Obstetrician and Gynaecologist

Dr Swatika Kumari

Obstetrician and Gynaecologist

19 Years • MBBS, DGO, DNB Obstetrics & Gynaecology

Nashik

Apollo 24|7 Clinic - Maharashtra, Nashik

726

Dr. Alapati Jyotsna, Obstetrician and Gynaecologist

Dr. Alapati Jyotsna

Obstetrician and Gynaecologist

4 Years • MBBS MS Obstetrics and Gynaecology

Visakhapatnam

Apollo 24|7 Clinic - Andhra Pradesh, Visakhapatnam

605

Dr. Mona Yadav, Obstetrician and Gynaecologist

Dr. Mona Yadav

Obstetrician and Gynaecologist

19 Years • MBBS, MD (Obstetrics & Gynaecology)

Dombivli

Nulife multispeciality, Dombivli

700

Understanding the Results

The results will show whether the levels of AFP, hCG, and estriol are normal, high, or low.

  • Normal levels – Lower risk of abnormalities.
  • Abnormal levels – May indicate a higher risk, but does not mean your baby definitely has a problem.

If results are concerning, your doctor may suggest further tests like:

  • Ultrasound – To check the baby’s growth.
  • Amniocentesis – A diagnostic test that examines amniotic fluid.
  • Non-Invasive Prenatal Testing (NIPT) – A more advanced blood test for genetic screening.

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What If the Results Are Abnormal?

First, don’t panic. An abnormal result does not always mean there’s a problem. Many women with unusual results go on to have perfectly healthy babies.

Your doctor will guide you on the next steps, which may include:

  • Repeat testing (sometimes levels vary).
  • Detailed ultrasound to check the baby’s development.
  • Genetic counseling to understand risks and options.

Lifestyle Tips for a Healthy Pregnancy

While tests like the Triple Marker help monitor your baby’s health, maintaining a healthy lifestyle is equally important:

  • Eat a balanced diet – Include fruits, vegetables, whole grains, and proteins.
  • Take prenatal vitamins – Especially folic acid, which helps prevent neural tube defects.
  • Avoid smoking and alcohol – These can harm your baby’s development.
  • Stay active – Gentle exercises like walking or prenatal yoga are great.
  • Manage stress – Practice relaxation techniques like deep breathing.

When to Consult a Doctor?

If you have concerns about your pregnancy or test results, don’t hesitate to speak with your doctor. Early detection and proper care can make a big difference.

Need to book a Triple Marker Test or consult a specialist?

You can easily schedule a test or appointment through Apollo 24|7 for expert guidance and care.

Final Thoughts

The Triple Marker Test is a helpful screening tool that provides valuable insights into your baby’s health. While it can be nerve-wracking to wait for results, remember that most pregnancies progress normally. Stay informed, follow your doctor’s advice, and take good care of yourself.

Consult a Gynaecologist for Personalised Advice

Dr. Priyanka Surisetty, Obstetrician and Gynaecologist

Dr. Priyanka Surisetty

Obstetrician and Gynaecologist

8 Years • MBBS, DGO

Visakhapatnam

Apollo 24|7 Clinic - Andhra Pradesh, Visakhapatnam

660

Dr Swatika Kumari, Obstetrician and Gynaecologist

Dr Swatika Kumari

Obstetrician and Gynaecologist

19 Years • MBBS, DGO, DNB Obstetrics & Gynaecology

Nashik

Apollo 24|7 Clinic - Maharashtra, Nashik

726

Dr. Alapati Jyotsna, Obstetrician and Gynaecologist

Dr. Alapati Jyotsna

Obstetrician and Gynaecologist

4 Years • MBBS MS Obstetrics and Gynaecology

Visakhapatnam

Apollo 24|7 Clinic - Andhra Pradesh, Visakhapatnam

605

Dr. Mona Yadav, Obstetrician and Gynaecologist

Dr. Mona Yadav

Obstetrician and Gynaecologist

19 Years • MBBS, MD (Obstetrics & Gynaecology)

Dombivli

Nulife multispeciality, Dombivli

700

Consult a Gynaecologist for Personalised Advice

Dr. Priyanka Surisetty, Obstetrician and Gynaecologist

Dr. Priyanka Surisetty

Obstetrician and Gynaecologist

8 Years • MBBS, DGO

Visakhapatnam

Apollo 24|7 Clinic - Andhra Pradesh, Visakhapatnam

660

Dr Swatika Kumari, Obstetrician and Gynaecologist

Dr Swatika Kumari

Obstetrician and Gynaecologist

19 Years • MBBS, DGO, DNB Obstetrics & Gynaecology

Nashik

Apollo 24|7 Clinic - Maharashtra, Nashik

726

Dr. Alapati Jyotsna, Obstetrician and Gynaecologist

Dr. Alapati Jyotsna

Obstetrician and Gynaecologist

4 Years • MBBS MS Obstetrics and Gynaecology

Visakhapatnam

Apollo 24|7 Clinic - Andhra Pradesh, Visakhapatnam

605

Dr. Asha Rani Singh, Obstetrician and Gynaecologist

Dr. Asha Rani Singh

Obstetrician and Gynaecologist

24 Years • MBBS DGO

Delhi

Dr Asha Rani Singh Clinic, Delhi

875

Dr. Mona Yadav, Obstetrician and Gynaecologist

Dr. Mona Yadav

Obstetrician and Gynaecologist

19 Years • MBBS, MD (Obstetrics & Gynaecology)

Dombivli

Nulife multispeciality, Dombivli

700

Consult a Gynaecologist for Personalised Advice

Dr. Priyanka Surisetty, Obstetrician and Gynaecologist

Dr. Priyanka Surisetty

Obstetrician and Gynaecologist

8 Years • MBBS, DGO

Visakhapatnam

Apollo 24|7 Clinic - Andhra Pradesh, Visakhapatnam

660

Dr Swatika Kumari, Obstetrician and Gynaecologist

Dr Swatika Kumari

Obstetrician and Gynaecologist

19 Years • MBBS, DGO, DNB Obstetrics & Gynaecology

Nashik

Apollo 24|7 Clinic - Maharashtra, Nashik

726

Dr. Alapati Jyotsna, Obstetrician and Gynaecologist

Dr. Alapati Jyotsna

Obstetrician and Gynaecologist

4 Years • MBBS MS Obstetrics and Gynaecology

Visakhapatnam

Apollo 24|7 Clinic - Andhra Pradesh, Visakhapatnam

605

Dr. Asha Rani Singh, Obstetrician and Gynaecologist

Dr. Asha Rani Singh

Obstetrician and Gynaecologist

24 Years • MBBS DGO

Delhi

Dr Asha Rani Singh Clinic, Delhi

875

Dr. Mona Yadav, Obstetrician and Gynaecologist

Dr. Mona Yadav

Obstetrician and Gynaecologist

19 Years • MBBS, MD (Obstetrics & Gynaecology)

Dombivli

Nulife multispeciality, Dombivli

700

Get Your Health Assessed

18750(₹25000)25% off

circle iconMember price
₹15000

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